hello,
i have to do invitro of dietary fiber, my sample is wheat bran, if any one have knowledge related to this please guide me
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how to to run an assay of dietary fiber in vitro?
Started by Shahid Ahmed, Aug 14 2016 11:51 PM
please help me
2 replies to this topic
#2
bob1
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Posted 15 August 2016 - 07:38 PM
No idea, however, dietary fiber is usually considered to be long chain, indigestible (but not insoluble) sugars. So you could dissolve the food in water/buffer, digest out the short chain sugars (e.g use amylase) then measure what is left (HPLC??)
#3
mdfenko
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Posted 16 August 2016 - 05:00 AM
amylase won't work if the fiber is cellulose, but cellulase should work.
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