If the primer is dimer,it's ok?
Posted 19 August 2004 - 03:45 PM
Posted 20 August 2004 - 08:07 PM
could you explain a little more about your problem...
Edited by SVTX, 20 August 2004 - 08:07 PM.
Posted 21 August 2004 - 12:10 AM
1) either re-order your primer
2) perform the PCR with the primers & if you are not getting contaminating bands, don't worry.
Posted 22 August 2004 - 09:56 PM
I guess it depends on how much of the sequence dimerises. If it's a lot then you may not have any free primer for the PCR reaction to proceed, so you won't get a product.
Posted 23 August 2004 - 01:12 AM
If time is not a factor in your experiment i would recommend that you try out the primers that you have allready ordered first before ordering a new one. Sometimes having primer-dimers aren't that bad , you can circumvent it by increasing the PCR temperature slightly to help destabilize the primer-dimers.
Posted 23 August 2004 - 09:38 PM