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How to prepare mice colon sample for ELISA

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#1 richie2048



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Posted 11 August 2016 - 11:42 AM

Hi Guys, 


I am planning on measuring inflammatory cytokines in mice colon sample. Because intestines contains quite a lot of proteases including some of the more unusual ones, which aren't covered by normal protease inhibitor cocktails. Do you add some other specific proteases to inhibit protein degradation? 



What is your colon tissue extraction buffer recipe? 


Thank you,


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