I was planning to perform MTT assay with U937 suspension culture. I am very confused about what should I do once I add MTT. Will it be wise to pellet the cells after MTT incubation and removing the supernatant, before redissolving them in DMSO, for taking the final reading. For adherent cells I remove the extra MTT after incubation and add DMSO to take the final reading.
Looking forward to suggestions.