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MTT assay for suspension cell culture

Started by Prersha, Aug 06 2016 09:34 AM

MTT suspension culture

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#1 Prersha

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Posted 06 August 2016 - 09:34 AM

Hi there.

I was planning to perform MTT assay with U937 suspension culture. I am very confused about what should I do once I add MTT. Will it be wise to pellet the cells after MTT incubation and removing the supernatant, before redissolving them in DMSO, for taking the final reading. For adherent cells I remove the extra MTT after incubation and add DMSO to take the final reading.

Looking forward to suggestions.

Thanks.

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