Google Sign in options
Remember me
This is not recommended for shared computers

Sign in anonymously
Don't add me to the active users list

Privacy Policy
Sign In
Create Account

Javascript Disabled Detected

You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality.

Submit your paper to J Biol Methods today!

MTT assay for suspension cell culture

Started by Prersha, Aug 06 2016 09:34 AM

MTT suspension culture

Please log in to reply

No replies to this topic

#1 Prersha

member

Members
2 posts

0
Neutral

Posted 06 August 2016 - 09:34 AM

Hi there.

I was planning to perform MTT assay with U937 suspension culture. I am very confused about what should I do once I add MTT. Will it be wise to pellet the cells after MTT incubation and removing the supernatant, before redissolving them in DMSO, for taking the final reading. For adherent cells I remove the extra MTT after incubation and add DMSO to take the final reading.

Looking forward to suggestions.

Thanks.

Back to MTT, Proliferation and Cytotoxicity Assay

Also tagged with one or more of these keywords: MTT, suspension culture

Protocols and Techniques Forums → Tissue and Cell Culture → MTT assay reading Started by Mustafa84, 26 Nov 2014 MCF-7, MTT, Cell line and 1 more...	1 reply 1,545 views	bob1 26 Nov 2014
Protocols and Techniques Forums → General Lab Techniques → Interpretation on absorbance reading from 96 well MTT assay Started by Frazermb, 23 Oct 2013 MTT, 96 well, absorbance, assay	0 replies 1,346 views	Frazermb 23 Oct 2013
Protocols and Techniques Forums → Immunology → MTT assay trouble shooting Started by Gunalan, 02 Apr 2013 Mtt	1 reply 1,620 views	antitoxin 16 Oct 2013
Protocols and Techniques Forums → Cell Biology → MTT, Proliferation and Cytotoxicity Assay → Large variations in MTT Started by hanazawayien, 18 Jan 2013 MTT	3 replies 1,864 views	Tabaluga 13 Feb 2013
Protocols and Techniques Forums → Tissue and Cell Culture → How to graph or plot MTT assay data Started by hidayahhh, 01 Jan 2013 data, plot, graph, MTT	10 replies 3,046 views	Peniel 12 Mar 2013