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Gel Electrophoresis: Gel Poured With Water


Best Answer bob1, 29 July 2016 - 07:23 PM

Other way round - heat is a byproduct of resistance.... less resistance, less heat. The gel heats up because there is limited conductivity, so greater resistance.

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#1 djvan

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Posted 29 July 2016 - 01:04 PM

Hello,

 

I'm in the process of training a recent college grad, and they accidentally made the mistake of pouring an agarose gel with water instead of buffer.  As expected, their gel overheated and they lost their DNA.

 

I started reading online as to why this happens, and couldn't find any scientific explanation.  So, can anyone explain why a gel cast with water, and run in a chamber with 1X TBE, conducts electricity better (I assume this is where the heat is coming from) than a gel cast with buffer?

 

Thanks!



#2 bob1

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Posted 29 July 2016 - 07:23 PM   Best Answer

Other way round - heat is a byproduct of resistance.... less resistance, less heat. The gel heats up because there is limited conductivity, so greater resistance.



#3 djvan

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Posted 30 July 2016 - 09:12 AM

Thanks Bob.  Electricity/current never was my forté.






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