Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

methylation primers


  • Please log in to reply
1 reply to this topic

#1 robertstpr

robertstpr

    member

  • Members
  • Pip
  • 1 posts
0
Neutral

Posted 19 August 2004 - 04:05 AM

Hello,
I have just statrted a project to look at methylation of a candidate gene. I have used the methprimer program to design 12 M and U primers. However, none of the primers seems to work on my genomic DNA. The DNA is quite pure (OD ratio 1.7), and intact and other primers amplify OK. I have tried different taq systems and temp/Mg.

Is this normal for so many primers not to work?

Thanks

#2 pcrman

pcrman

    Epigenetist

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 1,165 posts
68
Excellent

Posted 23 August 2004 - 08:57 AM

May I ask if or not you have modified your DNA with sodium bisulfite because you said "work on your genomic DNA"?

You said none of the 12 primer sets do not work on your DNA. Although sometimes primers may fail, other factors such as DNA template and PCR conditions may also contribute to PCR failure.




Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.