second strand cDNA
Posted 19 August 2004 - 01:32 AM
Please, share with me.
(There are a loads of first strand kit at manufacturers, but I never find second strand.)
Posted 22 August 2004 - 10:10 PM
Posted 22 August 2004 - 11:24 PM
Thanks stratagene web site.
Posted 22 May 2009 - 03:51 AM
I guess no protocol is needed as you obtain first cDNA strand, you can use that first cDNA as template in PCR reaction. Do it as a normal PCR. It works.
I am having the same question . I would like to know, if do it like the normal PCR, what type of primer shall I use? I need to have the full length of the cDNA (if possible) to be amplified.
Kits seems too expensive and I am doing for trial. May I know if anyone out there been doing this, and can send me some suggestion and advice?
Thanks in advance.