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second strand cDNA


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4 replies to this topic

#1 blasko

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Posted 19 August 2004 - 01:32 AM

Do you have a second strand cDNA protocol?
Please, share with me.
(There are a loads of first strand kit at manufacturers, but I never find second strand.)


Thanks.

#2 aj_xy999

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Posted 19 August 2004 - 09:56 PM

try: http://www.stratagen...ct.aspx?pid=609

#3 oddie

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Posted 22 August 2004 - 10:10 PM

I guess no protocol is needed as you obtain first cDNA strand, you can use that first cDNA as template in PCR reaction. Do it as a normal PCR. It works.

#4 blasko

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Posted 22 August 2004 - 11:24 PM

I need second strand DNA, because after sythesis I would like to digest by RE.

Thanks stratagene web site.

#5 cheerioet

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Posted 22 May 2009 - 03:51 AM

I guess no protocol is needed as you obtain first cDNA strand, you can use that first cDNA as template in PCR reaction. Do it as a normal PCR. It works.



I am having the same question :) . I would like to know, if do it like the normal PCR, what type of primer shall I use? I need to have the full length of the cDNA (if possible) to be amplified.

Kits seems too expensive and I am doing for trial. May I know if anyone out there been doing this, and can send me some suggestion and advice?

Thanks in advance.




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