I'm facing the "black dot contamination" problem in my stable cell line. The problem is that I cannot throw this culture away so I started to use the antibiotics (Cipro/Piper/Tazo, 10 ug/ml each + Pen/Strep) described in this paper http://www.ncbi.nlm....les/PMC2849847/.
(*stable cell lines were made by transfection of a sleeping beauty transposon vector (pSBTet-BP) inducible by doxycycline with a Blue Protein for visualizing Puromycin positive clones).
Strangely, after 1-3 days of treatment, most of the blue fluorescence of my positive stable cell line pool started to disappear!
Do guys know if Ciprofloxacin and/Piperacillinlin and/or Tazobactam can interfere in gene expression or it just decreasing cell fluorescence for no reason?
Thank you in advance!