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site directed mutagenesis

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#1 SF_HK



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Posted 27 June 2016 - 12:20 AM



I'm new to site directed mutagenesis and would appreciate all the help. 


I need to perform a single gene mutation. The antibody against my protein target is Ser552. I want to change the Ser552 to another amino acid, I assume Ser552 means serine at position 552 from the first amino acid. But the antibody data sheet states the serine at a much different position (i.e. 853), much further down the sequence. am'I missing something?

#2 bob1


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Posted 06 July 2016 - 06:38 AM

Are there splice variants for this gene? Or it could be that you are looking at a different species sequence, or the supplied sequence is wrong (mis-translated or starting from a different ATG).

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