I seem to have serious trouble with p-creb blotting... I have tried numeorus protocols and using different Abs but I either get no signal at all or multiple bands which are pretty close and make it hard to really figure out what band actually represents p-creb. I would appreciate if someone could take a look at the attached film and help me figure out which one of the bands is my protein of interest. I've been using cell signal p-creb antibody which is suppose to be 43 kDa..ANY help or comment is welcomed since I am at a point where I'm pulling my hair out
Edited by Tamar Tobi, 15 June 2016 - 09:32 AM.