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LR white resin and H&E staining


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#1 snd

snd

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Posted 14 June 2016 - 07:58 PM

I have embedded a sample in LR white resin. Then, sectioned it (thickness = 5 microns), and floated it in water on a microscope slide. Then, I've left it on a hot plate about 60 C to dry. H&E staining was done following that. However, I have a problem where all or majority of sections fall off by the time I even get to the eosin stain. I tried different things: egg albumin adhesive, using 40% acetone instead of water to float sections, polysine-coated adhesion slides and then baking at 65 C for 20 minutes (even for longer times, up to 40 minutes). Sometimes, if lucky, there is one or couple of sections that stick all the way through the staining process. However, I need all of them to stick. 

Does anyone have more experience with LR white resin and H&E staining? Do you have any advice or suggestions?






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