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cell viability assay for caco-2 and pbmc

cell viability assay mts assay mtt assay caco-2 pbmc

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#1 prollic

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Posted 06 June 2016 - 07:08 AM

Hi. I am interested to measure the cell viability of Caco-2 and human PBMCs.

 

1. Have anyone tried before using MTT/MTS/ATP assays/Neutral Red to measure cell viability of Caco-2? Is MTT/MTS assays more sensitive than NR?

2. I will be growing the cells for ~1 week and stimulating the cells for ~2 days in 24-well plates. Do I have to trypsinize the cells, count cells, and seed on 96 well plate? *Do anyone have any protocol for assays with Caco-2? 

3. I read that PBMCs will produce low readings using MTT assays. Do you have any recommendations? Will negative staining with PI/7-AAD suffice?

 

Thanks. 



#2 JJoanna

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Posted 28 June 2016 - 07:40 PM

protocol for assays with Caco-2:

http://static1.1.sqs...ztyDDdP4XH1Ff0=

 

Cell Viability Assay Kits:

http://www.creative-proteomics.com/search.aspx?q=Cell%20Viability







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