Hi. I am interested to measure the cell viability of Caco-2 and human PBMCs.
1. Have anyone tried before using MTT/MTS/ATP assays/Neutral Red to measure cell viability of Caco-2? Is MTT/MTS assays more sensitive than NR?
2. I will be growing the cells for ~1 week and stimulating the cells for ~2 days in 24-well plates. Do I have to trypsinize the cells, count cells, and seed on 96 well plate? *Do anyone have any protocol for assays with Caco-2?
3. I read that PBMCs will produce low readings using MTT assays. Do you have any recommendations? Will negative staining with PI/7-AAD suffice?