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CONJUGATION FREQUENCY


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#1 JBB

JBB

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Posted 24 May 2016 - 12:59 PM

Please help.

 

I know the equation which is number of transconjugants/number of donors but I don't know how to calculate either of these.

The example is:

E.coli at dilution 10-6 with 101 colonies. This is plated on a NA plate.(donor cells)

E.coli at dilution 10-6 with 101 colonies on NA, Streptomycin and ampicillin plate. (transconjugants)

 

It also says when working out donor cells to remember 0.1ml was plated and 1/10th would be donor cells.

 

Any help if greatly appreciated and please show working so I can answer the rest of them.

 

Thank you!

 






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