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reliable homemade mastermix with SybrgreenI for rt-pcr

mastermix RT-PCR Sybr-green

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#1 lorenzo



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Posted 23 May 2016 - 03:30 PM

hi there,


we spend a lot of money every year buying a pre-made mastermix 2x (DyNAmo HS SYBR Green qPCR Kit), for RT-PCR and Q-PCR.


do you guys have a reliable homemade master mix using Sybr green-I?

we have tons of Sybr greenI frozen, ready to be used. I'm looking ideally for something that can be made in big batches and then frozen (I'll use an aliquot when I need it).





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