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Can someone please explain this to me?


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#1 Questions

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Posted 20 May 2016 - 01:10 PM

Hi, I am looking for help and hope someone here with the appropriate knowledge can help me. Recently I took an oral swab hiv test and when I looked up what is inside the test stick I found this information and it confused me. Can someone please explain what these are and how they are made? I would really appreciate it!

 

HIV 1 & 2 Peptides/Panel Members (Defibrinated), GP-36 Peptide [Biotinylated](N/A), GP-41 Peptide [Biotinylated](N/A)

Recombinant Protein A

 

 

 



#2 Andrea Fortina

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Posted 20 June 2016 - 12:18 AM

HIV-1 and HIV-2 appear to package their RNA differently. HIV-1 binds to any appropriate RNA whereas HIV-2 preferentially binds to mRNA which creates the Gag protein itself. This means that HIV-1 is better able to mutate. HIV-2 is transmitted in the same ways as HIV-1: Through exposure to bodily fluids such as blood, semen, tears and vaginal fluids. Immunodeficiency develops more slowly with HIV-2.
HIV-2 is less infectious in the early stages of the virus
han with HIV-1. The infectiousness of HIV-2 increases as the virus progresses. Major differences include reduced pathogenicity of HIV-2 relative to HIV-1, enhanced immune control of HIV-2 infection and often some degree of CD4-independence. Despite considerable sequence and phenotypic differences between HIV-1 and 2 envelopes, structurally they are quite similar. Both membrane-anchored proteins eventually form the 6-helix bundles from the N-terminal and C-terminal regions of the ectodomain, which is common to many viral and cellular fusion proteins and which seems to drive fusion.
HIV-1 gp41 helical regions can form more stable 6-helix bundles than HIV-2 gp41 helical regions however HIV-2 fusion occurs at a lower threshold temperature (25°C), does not require Ca2+ in the medium, is insensitive to treatment of target cells with cytochalasin B, and is not affected by target membrane glycosphingolipid composition.

http://www.creativeb...search_gp36.htm

 

There are also instruction about protein A, but it seem not related to HIV, you can take as reference.

http://www.creativeb...h_Protein A.htm


Andrea Fortina

Genius only means hard-working for all one's life.

CreativeBiomart, Recombinant protein expert

 

 


#3 Zuzanny

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Posted 28 July 2017 - 08:32 PM

Andrea Fortina,thx for you infomation!biggrin.png biggrin.png


Edited by Zuzanny, 28 July 2017 - 08:36 PM.





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