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GFP transfection


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#1 SF_HK

SF_HK

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Posted 18 May 2016 - 07:42 AM

HI,

 

I'm planning to generate a stable cell line by co-transfection using a GFP plasmid and another plasmid contaning my gene of interest.  Ultimately we want to do live cell imaging of xenograft mice with the stable cell line.

 

My question is do I co-transect my cell line with equal amount of GFP and target plasmids or would the amount of GFP transfected would be less compared to the target plasmid?

 

Thanks






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