Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

GFP transfection

  • Please log in to reply
No replies to this topic

#1 SF_HK



  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 231 posts

Posted 18 May 2016 - 07:42 AM



I'm planning to generate a stable cell line by co-transfection using a GFP plasmid and another plasmid contaning my gene of interest.  Ultimately we want to do live cell imaging of xenograft mice with the stable cell line.


My question is do I co-transect my cell line with equal amount of GFP and target plasmids or would the amount of GFP transfected would be less compared to the target plasmid?



Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.