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Pericentromere specific probes?


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#1 Matan Yadgar

Matan Yadgar

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Posted 18 May 2016 - 05:59 AM

Hi all,

 

Im trying to do a FISH on DNA combing for pericentromeric regions.

 

As you may know, the human centromere is actually separated into two distinct regions: 1) centromeric region - which is involved in kinetochore binding 2) pericentromere region - a heterochromatic region which is involved in sister chromatid binding.

 

Im looking for pericentromere specific probes which do not overlap with any other region (centromeric or non centromeric).

 

Does anyone have a clue whether this kind of probes/BAC/HAC/anything else exist? if so - a link would be great!

 

Thank you!

 

Matan






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