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degenerate PCR


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#1 amira

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Posted 18 August 2004 - 06:52 AM

Hi,
I want to sequence my gene in different species. so I chose my degenerate primers and made my PCR, but I saw that just one primers can amplify my DNA. my primers are very unspecific, but I chose them in the very concerved part of my gene. I am sure that my gene is in this mix of amplification (lots of band) do you know a technic to extract my gene in this mix? or to have a more specific PCR...

#2 meshuga

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Posted 18 August 2004 - 11:04 AM

Hi, Amira,
Do you have a possibility to design primers for nested pcr? - it's a good way to fish out particular gene.

#3 amira

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Posted 18 August 2004 - 01:07 PM

Hi, Amira,
Do you have a possibility to design primers for nested pcr? -  it's a good way to fish out particular gene.

I did it, but my nested primers are degenerate too, so I still have lots of bands with it.




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