I'm designing an experiment to determine if a combination of five transcription factors is required for transcription of my target gene. I'd like to perform a western on my transfected cells in addition to the luciferase assay so that I can make sure that the transfection worked for all five factors. Normally, the lab does the assay in 96-well plates and doesn't confirm that the transfection worked, which I do not like. I'm looking for suggestions on how to go about this. Ideally, I'd like to transfect my cells (adherent) in a 6 well plate and use the same cells for luciferase and western. However, given that the lysis for the luciferase activity is done at room temperature, I don't have high hopes for a successful western. Any tips/strategies are greatly appreciated!