i have some questions about rna isolation from gram negative bacteria.
1- do i have to prepare the medium with depc threated water fro bacteria growth?
2- I am using thermo rneasy kit and i do all the santrifuges at room temp. instead of pelleting the culture. but I have no rna . shall i do the elution step at 4 C?
Does anyone who used thermo rneasy kit can explain the growing conditions and dilutions of the bactreial culture?
can i reuse my falcons for bacteria growing after autoclave?
thanx, have a nice day