I want to transfect my cells with siRNA-FITC labeled using electroporation.
Results were not good
comapny recommend to use conc equivalent to 100-250uM.
my siRNA already reconstituted in conc 50uM.
I want to make it more concentrated up to 200 uM
there is protocol to use ethanol/salt precipitation.
But electroporation manual doesn't recommend it due to traces of impurities that might cause cell death.
DO u have any suggestion for me.
I cannot increase the volume of used siRNA.
I must use 1ul.
Thanks in advance