Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
- - - - -

promoter activity of EMCV IRES

  • Please log in to reply
No replies to this topic

#1 sara.r



  • Active Members
  • PipPipPipPipPip
  • 95 posts

Posted 07 May 2016 - 09:21 PM

Hi everyone


We have recently constructed a promoterless reporter unit containing EMCV IRES-GFP-SV40 Poly A. After transfection into CHO cells we were surprised to see strong GFP expression in at least 70 percent of cells after 24 hours while we did not have any kind of promoter upstream of the unit. As far as I know IRES helps in cap independent translation. Is it possible that the IRES cause transcription of the GFP?



Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.