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Transfection with Lipofectamine and PEI, differences

transfectionLipofectamine PEI neuron polyethylenimine

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2 replies to this topic

#1 Coty Peretti

Coty Peretti


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Posted 04 May 2016 - 01:04 PM

Hi! I want to know what are the differences between PEI (polyethylenimine) and lipofectamine 2000. Is PEI more efficent?, are the mecanisms of transfection  the same? I want to transfect 10-14 DIV neurons with shRNA, what do you guys recomend?. Thank you!

#2 Andrea Fortina

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Posted 15 June 2016 - 01:51 AM

I'm sorry I cannot tell you the difference these two. Usually I use Lipo2000 for plasmids, but Lipo2000 works well with shRNA, too.

Andrea Fortina

Genius only means hard-working for all one's life.

CreativeBiomart, Recombinant protein expert



#3 bob1


    Thelymitra pulchella

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Posted 15 June 2016 - 04:55 AM

Lf2000 is a newer generation of transfection reagent. Compared with pei it is more efficient, but tends to be more toxic. In your situation I would try a range of transfection reagents. Personally I have used fugene and found it very good compared to lf2000, but that was in ordinary cells, not neurons.

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