I will design primers for test specific KD of gene in my cell line.
I read on internet, we should test primer efficiency by making STD curve and serial dilution of STD.
so can u give me ur advice?
for example, if my cell line specifically produced my tested gene, shall I use cDNA prepared from separation of mRNA as STD.
or shall I use other stander, like plasmid or some thing.
Do u have any other recommendation to make good STD curve