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Formula to calculate bacterial doubling time

growth curve bacteria doubling time formula

Best Answer Meg P. Anula, 18 April 2016 - 08:41 AM

I've solved it thanks.

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#1 Meg P. Anula

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Posted 18 April 2016 - 08:11 AM

Hi all,

 

I've checked the OD600 of my bacteria culture every hour and derive the doubling time manually from plotting graph by hand. 

 

But I wonder for my thesis writing, I probably needed use excel and formulas. 

 

I came across many formulas in this case but don't really understand them.

 

ie.

 

g=1/k, where g=doubling time and k is growth rate.

The formula for growth rate is k=logXt-logX0/0.301t.

 

Can anyone explain the formula for k?

 

Thx.



#2 Meg P. Anula

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Posted 18 April 2016 - 08:41 AM   Best Answer

I've solved it thanks.



#3 pito

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Posted 19 April 2016 - 07:44 AM

I've solved it thanks.

Maybe you should show how you solved it?

For people in the future with the same problem.

 

I am guessing you used the standard formula using the natural number e? Or how did you calculate it?

something like this:

cab93da0e7e89205c3d0a112b631e57d.png

If you don't know it, then ask it! Better to ask and look foolish to some than not ask and stay stupid.


#4 Meg P. Anula

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Posted 20 April 2016 - 07:14 AM

 

I've solved it thanks.

Maybe you should show how you solved it?

For people in the future with the same problem.

 

I am guessing you used the standard formula using the natural number e? Or how did you calculate it?

something like this:

cab93da0e7e89205c3d0a112b631e57d.png

 

 

No I stil used the same formula as I mentioned about:

g=1/k

k=logXt-logX0/0.301(t)

 

The problem was I forgot to change the time unit from hour to min and thought I got the wrong answer. Silly me laugh.png







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