I am looking for general techniques/additives to buffer to increase protein solubility and reduce precipitation. Our lab has had on and off issues with protein precipitation, and I figured if we could combat that that would increase our yields.
I know there are techniques for increasing solubility of transgenic proteins in E coli, such as co-expression of chaperonins, but I am looking for more in vitro, for any given protein.
So far I have that L-Arg and L-Glu can help at 50 mM.
What about Tween 20? Would that do anything?
Other buffers to try? pH and salt concentration would probably be protein specific, yeah?
I remember seeing a bunch of listed suggestions online somewhere but I can't find it again.