hi, all! Recently I have been trying to do a simple subcloning. After I digested my plasmid, I ran a gel to cut the insert out and used QIAGEN gel extraction kit to recover my insert. But I always got two band after recovery. One is the size of my insert and one is smaller.But both of them are very bright. I understood that sometime from the process of recovery, one will get some ssDNA.But that is always kind of a smear and never this bright. I don't understand why I keep getting two bands after recovery. Could the other band be something else instead of ssDNA? If it is ssDNA, how do I do to try to minimize it?
PS: my insert is about 6.2kb and the other unwanted band is always around 2kb.
Thanks a lot!!!
Edited by cytochrome, 17 August 2004 - 08:33 PM.