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TetA as a counter-selection marker

for lactic acid bacteria

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#1 rmbio

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Posted 05 April 2016 - 01:54 AM

I am planning of using tetA as a selection (tetracyclin resistance) as well as counter-selection (fusaric acid sensitivity) marker in lactic acid bacteria, mostly lactobacillus acidophilus. Although it is well established that it works for this dual function in E. coli (http://www.ncbi.nlm....cles/PMC108478/), I couldn't find any report  on usage of tetA for positive as well as negative selection in lactic acid bacteria (LAB). I can speculate that it should work in LAB as well.

Any comments/suggestions?

 

Thanks

 

Edit: Even Thymidine kinase (TK) (viral?) doesn't appear to have been used for lactic acid bacteria. Can anyone with experience in using TK as a negative selection marker comment on whether it will work in gram positive bacteria like lactic acid bacteria?


Edited by rmbio, 05 April 2016 - 02:23 AM.


#2 Andrea Fortina

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Posted 08 June 2016 - 12:55 AM

I also only use tetA as selection for E.coli.


Andrea Fortina

Genius only means hard-working for all one's life.

CreativeBiomart, Recombinant protein expert

 

 





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