I'm observing strange behavior of my Taqman probes during the very first cycles of PCR: fluorescence becomes negative first, on later cycles things going more usual, but that first pitch spoils the whole picture. (see picture)
I’ve tried to find out what is that but failed, anyone knows anything?
Thank you a lot in advance!
Edited by Rodriguez Ruslan, 01 April 2016 - 05:51 AM.