Are bacteria cells supposed to look smaller and not in their own shape when in fluorescence?
I did DAPI fluorescence stain to view my bacteria, I did not see any single bacillus like in the slide. I saw what supposed to be cell clump, but in it the cell that fluoresces looks so weird, it was like little spots. Overall, I just could not see bacillus shaped creatures. All I see was cell clumps (attached in pic here).
I did gram stain on my bacteria cells and confirm it is indeed bacillus.
I use x40 on both. (maximum on the microscope)
Attached are the pictures.