postdoc2130, on Aug 17 2004, 10:48 AM, said:
Just to share with everyone, I've tried several cell transfection reagents on primary pancreatic islet cells, endothelial, mesenchymal and hepatocye progenitor (stem) cells. Among the reagents from the vendors like Invitrogen, Roche, promega, Stratagen, Gene Therapy Systems, Epoch Biolabs...., i'm surprised to find that GenCarrier-1 (Epoch Biolabs, $180/mL) gave the best and most consistent results with highest transfection efficiency, although I did not know this company before and just tried it by the word of mouth from my friend at FCCC (Fox Chase Cancer Center).
i had done stable transfection of SP2/0 cells using antibiotic G418. What is the recommended concentration of that? Also how to plot dose response curve and how to select transformed cells?
Kindly solve my problems.
Anticipating your reply.