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Cytotoxicity of hoechst staining in overnight experiment

Cell Biology Hoechst cytotoxicity DNa staining

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#1 Badette

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Posted 18 March 2016 - 02:30 AM

Hi everyone,

 

Has anybody ever done an overnight microscopy acquisition of cells that were stained with hoechst just before starting the experiment? If so, did your cells survived the night? was there a big photo-bleaching of the Hoechst staining?

 

Thank you in advance for your answers :)

 

Juju



#2 Andrea Fortina

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Posted 16 May 2016 - 01:25 AM

Do you mean that you stained first and watched that at different points overnight?

For me, if I want to test cytotoxicity during a period, I will choose 4 or 6 checking points and use 4 or 6 parallel samples, not only one sample and watch all night.


Andrea Fortina

Genius only means hard-working for all one's life.

CreativeBiomart, Recombinant protein expert

 

 






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