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miRNA cloning


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#1 Skovic16

Skovic16

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Posted 18 March 2016 - 12:20 AM

Good day ladies and gentlemen,

I would like to ask something about plant pre-miRNA cloning.  If I already have the sequence of the pre-miRNA, is there a rule for the 100bp 5' and 3' flanking region?  What is the purpose of flanking and does the size or length of the flank really matter?

 

Thanks a lot






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