I have expressed allergen protein from dust mite, cysteine protease, in P.pastoris system by using 0.5% induction. At the first time that I set up the experiment, this protein can be expressed with good yield and low in backgroud. After I performed in large scale, nothing can be detected. I think it may be the effects of leakage, so I need the advice how I do.
Problem about gene leakage in P.pastoris
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