Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Use of fluorescent stain on IEF gels

IEF Fluorescent fixation

  • Please log in to reply
1 reply to this topic

#1 Missle

Missle

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 160 posts
27
Excellent

Posted 15 March 2016 - 10:00 AM

Hello.  I currently stain IEF gels (without fixation) with a combination of commassie/crocein stain and am interested in a fluorescent stain such as SYPRO Ruby or others.  Does anyone have any experience with using fluorescent stains with IEF gels and is fixation necessary?  Thanks!



#2 Michael Starr

Michael Starr

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 59 posts
1
Neutral

Posted 28 March 2016 - 02:44 PM

Yes, fixation is necessary.

 

http://www.bio-rad.c...006173B-wMS.pdf

(page 5)







Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.