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pGEM T Easy subcloning


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#1 delf0s

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Posted 13 March 2016 - 08:55 AM

Hi,

 

I'm trying to subclone from a pGEM T Easy vector to another one to express a protein. However, I need to find a vector that coincides with the RE sites. So far I have tried many without success. I was wondering if someone has an expression vector with His tags they recommend goes with the pGEM T Easy vector.



#2 bob1

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Posted 13 March 2016 - 11:54 PM

Why not PCR the insert with the desired RE sites (and HIS tag) included?






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