Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo

Target gene cloning


  • Please log in to reply
4 replies to this topic

#1 Skovic16

Skovic16

    member

  • Members
  • Pip
  • 4 posts
0
Neutral

Posted 07 March 2016 - 06:06 PM

How are you ladies and gentlemen,

 

I am quite a newbie in this field of miRNA and I have a question.  I am trying to clone a plant miRNA and its target gene and further overexpress them in a plant species but I am not sure if for the target gene I only have to focus on the ORF or include the 3' UTR and the 5'UTR.  If the target site of my miRNA is in the ORF of its target gene.

Thank you!



#2 bob1

bob1

    Thelymitra pulchella

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 6,507 posts
542
Excellent

Posted 13 March 2016 - 11:56 PM

Just the ORF should be fine.



#3 Jon Moulton

Jon Moulton

    Veteran

  • Active Members
  • PipPipPipPipPipPipPipPipPipPip
  • 147 posts
3
Neutral

Posted 16 March 2016 - 06:50 AM

You need the 3'-UTR if you want the target gene to be regulated by the miRNA.

 

Typically the miRNA response element (MRE) is in the 3'-UTR.  When the mRNA cyclizes before translation, the RISC bound to the 3'-UTR MRE is brought close to the 5'-cap and the assembling initiation complex.  It is the interaction of the RISC with an element of the initiation complex that supresses translation.


Jon D. Moulton
Gene Tools, LLC
www.gene-tools.com

#4 Skovic16

Skovic16

    member

  • Members
  • Pip
  • 4 posts
0
Neutral

Posted 18 March 2016 - 12:22 AM

Hey there,

Thanks a lot I greatly appreciate.



#5 Andrea Fortina

Andrea Fortina

    Enthusiast

  • Active Members
  • PipPipPipPipPip
  • 55 posts
1
Neutral

Posted 17 May 2016 - 08:20 PM

  I am trying to clone a plant miRNA and its target gene and further overexpress them in a plant species

 

I'm confused, you mean you want to clone miRNA and its target gene, these two both in a plant species?


Andrea Fortina

Genius only means hard-working for all one's life.

CreativeBiomart, Recombinant protein expert

 

 





Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.