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Quantifying bands on PAGE gel with ImageJ

ImageJ PAGE debugging protein quantification

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#1 Michael Starr

Michael Starr

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Posted 24 February 2016 - 11:17 AM

I am trying to use the rectangle, then Select First Lane, Select Final Lane option in ImageJ to quantify relative intensities of bands on a PAGE gel.

 

Here is an image of my problem: https://drive.google...iew?usp=sharing

 

So what doesn't work is I see the two rectangles on the gel image, but ImageJ says it doesn't recognize any rectangle selections.

 

Halp! >_<







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