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Stock solution

stock solution dilution

Best Answer Newbs, 22 February 2016 - 10:42 PM

Im confused because the chemokine website I bought it from says "20ug". I was told to make a 1000x stock solution of that and know what volume to add. For my assay, I need to use 1000ng/ml concentration which i would use from my stock solution once I have it made. 

 

But making the stock solution is throwing me off. 

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#1 Newbs

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Posted 22 February 2016 - 09:53 PM

I have to make the stock solution of powdered chemokines. I need to know what volume you need to resuspend the powdered chemokines for a 1000X stock.

 

For example one of the chemokines has a concentration of 20ug/ml, how would i make a 1000x stock?

 

Thanks!



#2 El Crazy Xabi

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Posted 22 February 2016 - 10:24 PM

1000x refers to the stock concentration respect to the concentration in the working solution, ie 1000 times higher concentration. If the final concentration of the chemokines you need is 20ug/mL, 1000x would be 20,000 ug/mL or 20 mg/mL



#3 Newbs

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Posted 22 February 2016 - 10:33 PM

So how would i know how much volume to add to the powdered chemokine?



#4 Newbs

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Posted 22 February 2016 - 10:42 PM   Best Answer

Im confused because the chemokine website I bought it from says "20ug". I was told to make a 1000x stock solution of that and know what volume to add. For my assay, I need to use 1000ng/ml concentration which i would use from my stock solution once I have it made. 

 

But making the stock solution is throwing me off. 



#5 El Crazy Xabi

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Posted 23 February 2016 - 04:36 PM

Correctme if I'm wrong:

your source chemokine (bought) = 20ug/mL

stock concentration desired = 1000x

working concentration (final) = 1000ng/mL

 

 

So your desired stock concentration would be 1000 ng/mL x 1000 = 1000000ng/ mL = 1 mg/mL stock = 1 ug/ uL

 

If the chemokine you bought is 20ug/mL you cannot make a stock of 1 mg/mL because your source is already 500 times more diluted. Are you sure about the units?







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