She short version:
WT factor V probe, HEX-ACC TGT ATT CCT CGC CT-BHQ-2
is not aligning to this gene sequence
The long version
Basically we did a Taqman end point genotyping experiment to to detect Leiden Factor V Disorder.
We are supposed to align the primers and probe/s to the F V gene sequence.
Here are the primers and probes
Forward primer 5′-GCC TCT GGG CTA ATA GGA CTA CTT C-3′
Reverse primer5′-TTT CTG AAA GGT TAC TTC AAG GAC AA 3’
The results of the experiment indicate that we had the WT variant.
I am using this site to align everything
The primers aligned perfectly, but the probe is not aligning. I tried to reverse it and translate it but still.
I would appreciate any help.
Edited by kabtq9s, 22 February 2016 - 07:55 PM.