Hi to all,
i need to quantify the intracellular metal absorbed in e.coli after treating.
I have used a total volume of 3ml, then i have pelletted them and washed with a buffer containing edta in order to chelate the unbound metal. eventually i have resuspend the pellet in 3 ml of water and digested the cells with other 3ml of digestion mixture before icp-ms analysis.
the results have been multiplied by two in order to take in account for the dilution factor, i'm wandering is should i have to correct also the values for the pellet, for example should i have to tonsider that the pellets have been resuspended in 3ml? so should i have to consider a dilution factor of three for these samples?