Jump to content

  • Log in with Facebook Log in with Twitter Log in with Windows Live Log In with Google      Sign In   
  • Create Account

Submit your paper to J Biol Methods today!
Photo
- - - - -

Primer sequences with M and Y - how to order?

Primers Mixed nucleotides

  • Please log in to reply
2 replies to this topic

#1 Ratnam

Ratnam

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 17 February 2016 - 07:48 PM

Hi,

    I have some primer sequences which I have to order. Got this from a paper, and these sequences have 'M' and 'Y' (mixed nucleotides) at some positions. How should I order? as it is with the M and Y or replace them appropriate nucleotides? Plz advice... Regards... Ratnam.



#2 phage434

phage434

    Veteran

  • Global Moderators
  • PipPipPipPipPipPipPipPipPipPip
  • 2,747 posts
304
Excellent

Posted 17 February 2016 - 08:27 PM

This probably depends on where you order from, but IDT, for example, will gladly synthesize with the M and Y in the sequence. You should be aware that the ratio of the bases in those positions are likely not exactly 50% due to inaccurate mixing of the reagents and differences in their reactivity during synthesis.



#3 Ratnam

Ratnam

    member

  • Members
  • Pip
  • 2 posts
0
Neutral

Posted 18 February 2016 - 01:45 AM

Thank for the prompt reply... that info was really useful...







Home - About - Terms of Service - Privacy - Contact Us

©1999-2013 Protocol Online, All rights reserved.