There's some unspecific protein bands in my chemiluminescence developed pvdf membrane. I believe those protein bands are from the bacteria as they are not the right size, and appeared even in negative control. And there's few band shown on my protein ladder as well. My target protein band, however, is no where to be found.
Aren't only one band (my target protein) supposed to manifest as I am using the chemiluminescence treatment with specific primary antibody?
I am confused.