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[Ca2+] in a muscle lysate

calcium assay muscle lysate

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#1 Eaglefan

Eaglefan

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Posted 13 February 2016 - 08:23 PM

Hello,

 

We would normally use single muscle fibers to determine intracellular calcium, but the only sample we have is pre-homogenized lysate (RIPA buffer). Is there any method that we could use as a proxy for intracellular calcium levels?

 

Any help would be greatly appreciated!
 







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