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SDS-PAGE vertical lane streaking

SDS-PAGE Lane streaking

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#1 Missle

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Excellent

Posted 10 February 2016 - 10:06 AM

Hello.  I'm running total cell protein samples in a 4-20% Mini-Protean TGX gels.  The center of each lane/sample contains a vertical streak/line.  Is this from interference of genomic DNA?  I was able to decrease the amount of genomic DNA in my samples (so they had no issues when using a pipettte), which decreased the intensity of the line but did not remove it.  It is not a huge issue for me but one I would like to understand better and thought someone on this forum would likely know the answer.

 

Thank you!

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#2 GNANA

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Good

Posted 10 February 2016 - 12:34 PM

It looks DNA to me.  you might need to play around your lysis buffer detergent concentration inorder to keep them mild.  or if you need the nuclear proteins also use nuclease like Benzonase to eliminate the DNA completely.


I always had an alternate hypothesis....






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