1. Have anyone experienced using auto-exposure setting in Biorad's ChemiDoc XRS and found that it took longer than 10 min but still no results shown?
Does that mean there's nothing on the membrane?
2. I plan to reprobe the same membrane with lower dilution of primary antibody. But I am afraid since the membrane has been dried for more than half an hour in the ChemiDoc's darkroom hood while I was testing the exposure time. Would there any effect on the protein that makes it unable to be reprobed?
Thanks in advance.
Edited by Meg P. Anula, 05 February 2016 - 07:37 AM.