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Storing membrane after antibody incubation

secondary antibody HRP membrane store condition

Best Answer mdfenko, 04 February 2016 - 06:49 AM

i would leave out the tween, just use tbs or pbs with blocking agent to store the membrane.

 

i still wouldn't leave it for too long but should be stable enough for a few days.

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#1 Meg P. Anula

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Posted 03 February 2016 - 07:18 AM

Hi guys,

 

I have incubated my pvdf membrane with HRP-conjugated secondary antibodies and washed with TBST, but due to some problem I plan to leave it for few days without developing it with HRP substrate. 

 

Can I soak the membrane in TBST and kept at 4c for few days before I developing the membrane?

Or do I have to repeat primary and secondary antibodies incubation again using the same membrane after few days?

 

Please advise on proper storage of the membrane if anyone knows.

 

Thanks in advance.


Edited by Meg P. Anula, 03 February 2016 - 07:59 AM.


#2 Missle

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Posted 03 February 2016 - 08:30 AM

I don't know what to recommend for proper storage but I wouldn't trust any results you get from leaving the primary and secondary antibodies on for such an extended period of time.  I would expect significantly reduced signal.



#3 mdfenko

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Posted 04 February 2016 - 06:49 AM   Best Answer

i would leave out the tween, just use tbs or pbs with blocking agent to store the membrane.

 

i still wouldn't leave it for too long but should be stable enough for a few days.


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