I want to start a ELISA assay to measurement released dopamine of SH-SY5Y cells after a stimulation with an agonist of nicotinic receptor.
I don´t know the best way to preserve extracellular dopamine of supernatant, could someone tell me what is the best buffer to do it?
To preserve dopamine, Should supernatant be acidify to avoid dopamine degradation? I have found a buffer with 0,2 mM sodium ascorbate although they don´t indicate which its pH, but I don´t know whether it is the right thing or I must use a acylation buffer.
And, could you recommend me a ELISA kit or a reliable comercial company where I can looking for?
I would be very grateful someone helps me, please.