I want to check whether if an interaction between two proteins is affected by cell cycle. I'll like to perform an IP with cultures enriched in S-phase, G2/M and G0/G1 phases. I've been reading protocols and I've found that serum starvation is easy to perform (2 days in serum-free DMEM and cells should be in G0). My concern is that I've seen this kind of protocol for HEK293 cells and I'll be using the HEK293T.
Someone has used serum deprivation as a way to synchronise HEK293T? If it can be used could you facilitate the protocol? Also, how many hours after adding DMEM with serum I have to wait to get cells in S-phase and G2-phase?
Thank you in advance,