I would like to know if you think iTRAQ is a good technique for this objective:
I wish to perform a time course of the protein profile in cells treated with a drug in the following time points: 0, 2, 4, 8, 12, 24, 48h.
I am afraid that iTRAQ will be too expensive and my supervisor told me to look for alternatives. I can try custom made ELISAs but these are not as broad as proteomic analysis, and I think it is expensive too...
What do you recommend iTRAQ or ELISA?